Cameron IFNG

Investigation Details

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GEO Submission: Public - RNAseq

GEO Accession: GSE299807

Submitted: 2025-06-13 Released: 2025-12-17
Total human lung cells from n=9 donors were stimulated in vitro for 18 hours with peptide pools specific for either influenza virus or cytomegalovirus (CMV), or left unstimulated. Antigen-specific CD8⁺ T cells were identified using activation-induced markers (CD25 and CD137) and isolated by FACS for bulk RNA sequencing. A PMA/ionomycin-stimulated sample was included as a positive control to set sorting gates. Unstimulated memory CD8⁺CD69⁺ T cells were also sorted as a comparison population.
GEO Submission: Public - scRNAseq

GEO Accession: GSE299811

Submitted: 2025-06-13 Released: 2025-12-17
scRNAseq of airway epithelial cells comparing three stimulation conditions. Total epithelial cells were divided into subsets based on key transcription factors post sequencing.
GEO Submission: Public - RNAseq

GEO Accession: GSE299808

Submitted: 2025-06-13 Released: 2025-12-17
ALI cultures of NHBEs were infected apically with H1N1 (A/Netherlands/602/2009) at MOI 0.1. IFNγ (100 ng/mL) was added basolaterally 4 hours post-infection. At 18 hours post-infection, epithelial cells were separated by FACS into basal, secretory, and ciliated subsets for bulk RNA sequencing. Samples were collected from three treatment groups (uninfected, infection only, infection + IFNγ), with all three epithelial subsets analyzed per group.
Investigation Information
Name: Cameron IFNG
Principal Investigator: Jake Kohlmeier
Institution: None
Contact Email: clrobi3@emory.edu
Created: 2025-08-21 16:57
Last Updated: 2025-12-21 21:38
Status: Completed
Description

Cameron Robinson's PhD work on Interferon gamma effects in epithelial crosstalk to lung CD8 TRM

Associated Publication
Human lung CD8(+) tissue-resident memory T cell-derived interferon-γ orchestrates subset-specific antiviral programming in airway epithelial cells.

Authors: Mattingly CLR, Jimenez AR, Williams ME et al.

Journal: Immunity (2025)

PMID: 41308652

Quick Statistics

2

Studies

2

Assays

12

Samples
Associated Studies
New Study
Study Name Type Organism Assays Samples Created Actions
Cameron_IFNG_apr23 Single-cell RNA-seq Homo sapiens 1 4 2025-08-21
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Cameron_scRNA_Oct2023 Single-cell RNA-seq Homo sapiens 1 8 2025-08-22
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